Characterization of Escherichia coli dinJ - yafQ toxin - antitoxin system : 3 insights from mutagenesis
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چکیده
2 Characterization of Escherichia coli dinJ-yafQ toxin-antitoxin system: 3 insights from mutagenesis data 4 5 Julija Armalytė, Milda Jurėnaitė , Gina Beinoravičiūtė, Justinas Teišerskas, and Edita 6 Sužiedėlienė* 7 8 Department of Biochemistry and Biophysics, Faculty of Natural Sciences of Vilnius 9 University, Čiurlionio 21, LT-03101, Vilnius, Lithuania; 10 11 12 13 Running title: characterization of E. coli dinJ-yafQ toxin-antitoxin system 14 15 *Correspondence: 16 Edita Sužiedėlienė, Department of Biochemistry and Biophysics, Faculty of Natural Sciences, 17 Vilnius University, Čiurlionio 21, Vilnius LT-03101, Lithuania. 18 Tel.: +370 5 2398230; Fax: +370 5 2398231; e-mail: [email protected] 19 20 21 Copyright © 2012, American Society for Microbiology. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.06104-11 JB Accepts, published online ahead of print on 13 January 2012
منابع مشابه
Characterization of Escherichia coli dinJ-yafQ toxin-antitoxin system using insights from mutagenesis data.
Escherichia coli dinJ-yafQ operon codes for a functional toxin-antitoxin (TA) system. YafQ toxin is an RNase which, upon overproduction, specifically inhibits the translation process by cleaving cellular mRNA at specific sequences. DinJ is an antitoxin and counteracts YafQ-mediated toxicity by forming a strong protein complex. In the present study we used site-directed mutagenesis of YafQ to de...
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